Our lab is using new technologies to couple rapid identification of interesting genes with methods for studying the consequences of their expression in an organismic context. We have developed an efficient type of expression cloning of signal transduction intermediates that allows us to rapidly identify cDNAs encoding genes that engage a number of known transduction pathways.

In addition, we continue to work on methods for creating mutant cell lines that have lesions in signal transduction pathways, and on appropriate ways to uncover the genetic basis of those lesions.

We are also developing systems for rapid generation of mice bearing targeted disruptions of specific candidate genes. Many of the latter projects are directed at large-scale changes in the mouse genome, which will help us create better mouse models of human disease.

In conjunction with the CCIB chemistry and therapeutics group we have been working on new strategies to treat disease using novel biologicals and pharmaceuticals.