Map created at http://pga.mgh.harvard.edu/web_apps/web_map/start Editor at http://pga.mgh.harvard.edu/web_apps/dna_utilities.html
*** Sites protected by E. coli methylation are not shown in this map *** Eco57 | GGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCT 1 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 100 CCGCATTAGACGACGAACGTTTGTTTTTTTGGTGGCGATGGTCGCCACCAAACAAACGGCCTAGTTCTCGATGGTTGAGAAAAAGGCTTCCATTGACCGA TCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCT 101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 200 AGTCGTCTCGCGTCTATGGTTTATGACAGGAAGATCACATCGGCATCAATCCGGTGGTGAAGTTCTTGAGACATCGTGGCGGATGTATGGAGCGAGACGA Eco57 AlwN1 | BpuE1 | | | GAAGCCAGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGAGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGA 201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 300 CTTCGGTCAATGGTCACCGACGACGGTCACCGCTATTCAGCACAGAATGGCCCAACCTGAGTTCTCTATCAATGGCCTATTCCGCGTCGCCAGCCCGACT ApaL1 BseY1 | | ACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAG 301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 400 TGCCCCCCAAGCACGTGTGTCGGGTCGAACCTCGCTTGCTGGATGTGGCTTGACTCTATGGATGTCGCACTCGATACTCTTTCGCGGTGCGAAGGGCTTC Eci1 BciV1 BssS1 | | | GGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGT 401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 500 CCTCTTTCCGCCTGTCCATAGGCCATTCGCCGTCCCAGCCTTGTCCTCTCGCGTGCTCCCTCGAAGGTCCCCCTTTGCGGACCATAGAAATATCAGGACA Pme1 Drd1 BpuE1 Eci1 Dra1 | | | | CGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCAAGCTAGAGTTTAAAC 501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 600 GCCCAAAGCGGTGGAGACTGAACTCGCAGCTAAAAACACTACGAGCAGTCCCCCCGCCTCGGATACCTTTTTGCGGTCGTTGCGTTCGATCTCAAATTTG Ssp1 | TTGACAGATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAAAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTT 601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 700 AACTGTCTACTCTGTTATTGGGACTATTTACGAAGTTATTATAACTTTTTCCTTTTCATACTCATAAGTTGTAAAGGCACAGCGGGAATAAGGGAAAAAA amp > M S I Q H F R V A L I P F F Ale1 | GCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCAGAAGATCACTTGGGTGCGCGAGTGGGTTACATCGAACTGG 701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 800 CGCCGTAAAACGGAAGGACAAAAACGAGTGGGTCTTTGCGACCACTTTCATTTTCTACGTCTTCTAGTGAACCCACGCGCTCACCCAATGTAGCTTGACC amp > A A F C L P V F A H P E T L V K V K D A E D H L G A R V G Y I E L D Acl1 BpuE1 | Dra1 | | | ATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTCCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCG 801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 900 TAGAGTTGTCGCCATTCTAGGAACTCTCAAAAGCGGGGCTTCTTGCAAAGGGTTACTACTCGTGAAAATTTCAAGACGATACACCGCGCCATAATAGGGC amp > L N S G K I L E S F R P E E R F P M M S T F K V L L C G A V L S R Bcg1b Bcg1a | | TATTGATGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAATACTCACCAGTCACAGAAAAGCATCTTACGGATGGC 901 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1000 ATAACTACGGCCCGTTCTCGTTGAGCCAGCGGCGTATGTGATAAGAGTCTTACTGAACCAACTTATGAGTGGTCAGTGTCTTTTCGTAGAATGCCTACCG amp > I D A G Q E Q L G R R I H Y S Q N D L V E Y S P V T E K H L T D G Bts1 Bts1 | | ATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCG 1001 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1100 TACTGTCATTCTCTTAATACGTCACGACGGTATTGGTACTCACTATTGTGACGCCGGTTGAATGAAGACTGTTGATAGCCTCCTGGCTTCCTCGATTGGC amp > M T V R E L C S A A I T M S D N T A A N L L L T T I G G P K E L T A CTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCC 1101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1200 GAAAAAACGTGTTGTACCCCCTAGTACATTGAGCGGAACTAGCAACCCTTGGCCTCGACTTACTTCGGTATGGTTTGCTGCTCGCACTGTGGTGCTACGG amp > F L H N M G D H V T R L D R W E P E L N E A I P N D E R D T T M P BsrD1 Acl1 Eci1 | | | TGTAGCAATGGCAACAACGTTGCGAAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAACTAATAGACTGGATGGAGGCGGATAAAGTT 1201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1300 ACATCGTTACCGTTGTTGCAACGCTTTTGATAATTGACCGCTTGATGAATGAGATCGAAGGGCCGTTGTTGATTATCTGACCTACCTCCGCCTATTTCAA amp > V A M A T T L R K L L T G E L L T L A S R Q Q L I D W M E A D K V BsrD1 | GCAGGACCACTTCTGCGCTCGGCACTTCCGGCTGGCTGGTTTATTGCTGATAAATCAGGAGCCGGTGAGCGTGGGTCACGCGGTATCATTGCAGCACTGG 1301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1400 CGTCCTGGTGAAGACGCGAGCCGTGAAGGCCGACCGACCAAATAACGACTATTTAGTCCTCGGCCACTCGCACCCAGTGCGCCATAGTAACGTCGTGACC amp > A G P L L R S A L P A G W F I A D K S G A G E R G S R G I I A A L G Bmr1 | GGCCGGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACTACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTC 1401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1500 CCGGCCTACCATTCGGGAGGGCATAGCATCAATAGATGTGATGCCCCTCAGTCCGTTGATACCTACTTGCTTTATCTGTCTAGCGACTCTATCCACGGAG amp > P D G K P S R I V V I Y T T G S Q A T M D E R N R Q I A E I G A S BsaXa BsaXb BciV1 Nde1 | | | | ACTGATTAAGCATTGGTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGT 1501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1600 TGACTAATTCGTAACCATTCCTATTTAAAGACCATTCCTCCTGTGCATACCTTCACCCGTTCAACCCCTTCGGCATAGGCAACGACTTAGACCGTATACA amp > L I K H W * Spe1 | GGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGGCATAACTAGTGAGGCTC 1601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1700 CCCTCATATTCTGCGCGTCGCAGCGTAGTCCGTAAAAAAAGACGCGGTTACGTTTTTCCGGTAGGCAGTCCTACCGGAAAGCCGTATTGATCACTCCGAG Mfe1 Age1 EcoN1 | | | CGGTGCCCGTCAGTGGGCAGAGCGCACATCGCCCACAGTCCCCGAGAAGTTGGGGGGAGGGGTCGGCAATTGAACCGGTGCCTAGAGAAGGTGGCGCGGG 1701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1800 GCCACGGGCAGTCACCCGTCTCGCGTGTAGCGGGTGTCAGGGGCTCTTCAACCCCCCTCCCCAGCCGTTAACTTGGCCACGGATCTCTTCCACCGCGCCC Bsg1 Bmr1 Eci1 Acl1 | | | | | GTAAACTGGGAAAGTGATGTCGTGTACTGGCTCCGCCTTTTTCCCGAGGGTGGGGGAGAACCGTATATAAGTGCAGTAGTCGCCGTGAACGTTCTTTTTC 1801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 1900 CATTTGACCCTTTCACTACAGCACATGACCGAGGCGGAAAAAGGGCTCCCACCCCCTCTTGGCATATATTCACGTCATCAGCGGCACTTGCAAGAAAAAG Sac2 Xmn1 | | GCAACGGGTTTGCCGCCAGAACACAGGTAAGTGCCGTGTGTGGTTCCCGCGGGCCTGGCCTCTTTACGGGTTATGGCCCTTGCGTGCCTTGAATTACTTC 1901 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 2000 CGTTGCCCAAACGGCGGTCTTGTGTCCATTCACGGCACACACCAAGGGCGCCCGGACCGGAGAAATGCCCAATACCGGGAACGCACGGAACTTAATGAAG orf 2 < * K Pst1 Stu1 Afl2 BssS1 | | | | CACCTGGCTGCAGTACGTGATTCTTGATCCCGAGCTTCGGGTTGGAAGTGGGTGGGAGAGTTCGAGGCCTTGCGCTTAAGGAGCCCCTTCGCCTCGTGCT 2001 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 2100 GTGGACCGACGTCATGCACTAAGAACTAGGGCTCGAAGCCCAACCTTCACCCACCCTCTCAAGCTCCGGAACGCGAATTCCTCGGGGAAGCGGAGCACGA orf 2 < W R A A T R S E Q D R A E P Q F H T P S N S A K R K L L G K A E H BseY1 BpuE1 | Dra1 | | | TGAGTTGAGGCCTGGCCTGGGCGCTGGGGCCGCCGCGTGCGAATCTGGTGGCACCTTCGCGCCTGTCTCGCTGCTTTCGATAAGTCTCTAGCCATTTAAA 2101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 2200 ACTCAACTCCGGACCGGACCCGCGACCCCGGCGGCGCACGCTTAGACCACCGTGGAAGCGCGGACAGAGCGACGAAAGCTATTCAGAGATCGGTAAATTT orf 2 < K L Q P R A Q A S P G G R A F R T A G E R R D R Q K R Y T E L W K F BspM1 BfrB1 Bsg1 | | ATTTTTGATGACCTGCTGCGACGCTTTTTTTCTGGCAAGATAGTCTTGTAAATGCGGGCCAAGACGATCTGCACACTGGTATTTCGGTTTTTGGGGCCGC 2201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 2300 TAAAAACTACTGGACGACGCTGCGAAAAAAAGACCGTTCTATCAGAACATTTACGCCCGGTTCTGCTAGACGTGTGACCATAAAGCCAAAAACCCCGGCG orf 2 < N K I V Q Q S A K K R A L Y D Q L H P G L R D A C Q Y K P K Q P R Apa1 Sac2 PspOM | BseY1 EcoK Nsi1 BpuE1 | | | | | | | GGGCGGCGACGGGGCCCGTGCGTCCCAGCGCACATGCATGTTCGGCGAGGCGGGGCCTGCGAGCGCGGCCACCGAGAATCGGACGGGGGTAGTCTCAAGC 2301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 2400 CCCGCCGCTGCCCCGGGCACGCAGGGTCGCGTGTACGTACAAGCCGCTCCGCCCCGGACGCTCGCGCCGGTGGCTCTTAGCCTGCCCCCATCAGAGTTCG orf 2 < P R R R P G H T G A C M C T R R P P A Q S R P W R S D S P P L R L Fse1 Nae1 | BseY1 Sac1 | | | | TGGCCGGCCTGCTCTGGTGCCTGGCCTCGCGCCGCCGTGTATCGCCCCGCCCTGGGCGGCAAGGCTGGGAGCTCAAAATGGAGGACGCGGCGCTCGGGAG 2401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 2500 ACCGGCCGGACGAGACCACGGACCGGAGCGCGGCGGCACATAGCGGGGCGGGACCCGCCGTTCCGACCCTCGAGTTTTACCTCCTGCGCCGCGAGCCCTC orf 2 < S A P R S Q H R A E R R R T D G R G P R C P Q S S L I S S A A S P L BsrB1 Bpu10 BbvC1 Nar1 Bgl1 | | | | | AGCGGGCGGGTGAGTCACCCACACAAAGGAAAAGGGCCTTTCCGTCCTCAGCCGTCGCTTCATGTGACTCCACGGAGTACCGGGCGCCGTCCAGGCACCT 2501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 2600 TCGCCCGCCCACTCAGTGGGTGTGTTTCCTTTTCCCGGAAAGGCAGGAGTCGGCAGCGAAGTACACTGAGGTGCCTCATGGCCCGCGGCAGGTCCGTGGA orf 2 < A P P H T V W V F S F P R E T R L R R K M Xho1 Dra3 | | CGATTAGTTCTCGAGCTTTTGGAGTACGTCGTCTTTAGGTTGGGGGGAGGGGTTTTATGCGATGGAGTTTCCCCACACTGAGTGGGTGGAGACTGAAGTT 2601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 2700 GCTAATCAAGAGCTCGAAAACCTCATGCAGCAGAAATCCAACCCCCCTCCCCAAAATACGCTACCTCAAAGGGGTGTGACTCACCCACCTCTGACTTCAA Bgl1Eco57 BpuE1 | | | AGGCCAGCTTGGCACTTGATGTAATTCTCCTTGGAATTTGCCCTTTTTGAGTTTGGATCTTGGTTCATTCTCAAGCCTCAGACAGTGGTTCAAAGTTTTT 2701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 2800 TCCGGTCGAACCGTGAACTACATTAAGAGGAACCTTAAACGGGAAAAACTCAAACCTAGAACCAAGTAAGAGTTCGGAGTCTGTCACCAAGTTTCAAAAA Xba1 Xmn1 | Hind3| | Xho1 || | | TTCTTCCATTTCAGGTGTCGTGAAAAGCTTCTAGAGATCCCTCGACCTCGAGATCCATTGTGCTCTAAAGGAGATACCCGGCCAGACACCCTCACCTGCG 2801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 2900 AAGAAGGTAAAGTCCACAGCACTTTTCGAAGATCTCTAGGGAGCTGGAGCTCTAGGTAACACGAGATTTCCTCTATGGGCCGGTCTGTGGGAGTGGACGC Pvu2 BseY1 | BspM1| | BstX1 BspM1 BfrB1| | BbvC1 Bpu10 Nco1| Bsa1 Msc1 BseY1BfrB1 || | | | || | | | | GTGCCCAGCTGCCCAGGCTGAGGCAAGAGAAGGCCAGAAACCATGCCCATGGGGTCTCTGCAACCGCTGGCCACCTTGTACCTGCTGGGGATGCTGGTCG 2901 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 3000 CACGGGTCGACGGGTCCGACTCCGTTCTCTTCCGGTCTTTGGTACGGGTACCCCAGAGACGTTGGCGACCGGTGGAACATGGACGACCCCTACGACCAGC ENV > M P M G S L Q P L A T L Y L L G M L V A Nhe1 | CTTCCGTgctagccactgagaagctgtgggtgactgtgtactatggggtgcctgtgtggaaggaggccaccaccaccctgttctgtgcctctgatgccaa 3001 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 3100 GAAGGCAcgatcggtgactcttcgacacccactgacacatgataccccacggacacaccttcctccggtggtggtgggacaagacacggagactacggtt ENV > S V L A T E K L W V T V Y Y G V P V W K E A T T T L F C A S D A K ApaL1 Stu1 Ale1 | Dra3 Bgl1 | | | | | ggcctatgacactgaggtgcacaatgtgtgggccacccaggcctgtgtgcccactgaccccaacccccaggaggtggagctggtgaatgtgactgagaac 3101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 3200 ccggatactgtgactccacgtgttacacacccggtgggtccggacacacgggtgactggggttgggggtcctccacctcgaccacttacactgactcttg ENV > A Y D T E V H N V W A T Q A C V P T D P N P Q E V E L V N V T E N BspLU Nsi1 AlwN1 Eco57 Bsg1 | | | | | ttcaacatgtggaagaacaacatggtggagcagatgcatgaggacatcatcagcctgtgggaccagagcctgaagccctgtgtgaagctgacccccctgt 3201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 3300 aagttgtacaccttcttgttgtaccacctcgtctacgtactcctgtagtagtcggacaccctggtctcggacttcgggacacacttcgactggggggaca ENV > F N M W K N N M V E Q M H E D I I S L W D Q S L K P C V K L T P L C Bsu36 Bts1 | | gtgtgaccctgaactgcactgacctgaggaacaccaccaacaccaacaacagcactgccaacaacaacagcaactctgagggcaccatcaagggggggga 3301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 3400 cacactgggacttgacgtgactggactccttgtggtggttgtggttgttgtcgtgacggttgttgttgtcgttgagactcccgtggtagttcccccccct ENV > V T L N C T D L R N T T N T N N S T A N N N S N S E G T I K G G E Pst1 BsrG1 | | gatgaagaactgcagcttcaacatcaccaccagcatcagggacaagatgcagaaggagtatgccctgctgtacaagctggacattgtgagcattgacaat 3401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 3500 ctacttcttgacgtcgaagttgtagtggtggtcgtagtccctgttctacgtcttcctcatacgggacgacatgttcgacctgtaacactcgtaactgtta ENV > M K N C S F N I T T S I R D K M Q K E Y A L L Y K L D I V S I D N Pvu2 Ale1 Ale1 | | | gacagcaccagctacaggctgatcagctgcaacacctctgtgatcacccaggcctgccccaagatcagctttgagcccatccccatccactactgtgccc 3501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 3600 ctgtcgtggtcgatgtccgactagtcgacgttgtggagacactagtgggtccggacggggttctagtcgaaactcgggtaggggtaggtgatgacacggg ENV > D S T S Y R L I S C N T S V I T Q A C P K I S F E P I P I H Y C A P Bts1 Stu1 BsrD1 Eco57 Pvu2 ApaL1 | Bsg1 | | | | | | | | ctgctggctttgccatcctgaagtgcaatgacaagaagttctctggcaagggcagctgcaagaatgtgagcactgtgcagtgcactcatggcatcaggcc 3601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 3700 gacgaccgaaacggtaggacttcacgttactgttcttcaagagaccgttcccgtcgacgttcttacactcgtgacacgtcacgtgagtaccgtagtccgg ENV > A G F A I L K C N D K K F S G K G S C K N V S T V Q C T H G I R P AlwN1 Pvu2 | BbvC1 BseR1 BseY1 | | EcoN1| Bpu10 BseR1 | ApaL1 | | | || | | | | tgtggtgagcacccagctgctgctgaatggcagcctggctgaggaggaggtggtgatcaggtctgagaacttcactgacaatgccaagaccatcattgtg 3701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 3800 acaccactcgtgggtcgacgacgacttaccgtcggaccgactcctcctccaccactagtccagactcttgaagtgactgttacggttctggtagtaacac ENV > V V S T Q L L L N G S L A E E E V V I R S E N F T D N A K T I I V Bsg1 Bsg1 Ear1 | | | cacctgaatgagtctgtgcagatcaactgcaccaggcccaactacaacaagaggaagaggattcacattggccctggcagggccttctacaccaccaaga 3801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 3900 gtggacttactcagacacgtctagttgacgtggtccgggttgatgttgttctccttctcctaagtgtaaccgggaccgtcccggaagatgtggtggttct ENV > H L N E S V Q I N C T R P N Y N K R K R I H I G P G R A F Y T T K N Bts1 Bsu36 | | acatcattggcaccatcaggcaggcccactgcaacatcagcagggccaagtggaatgacaccctgaggcagattgtgagcaagctgaaggagcagttcaa 3901 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 4000 tgtagtaaccgtggtagtccgtccgggtgacgttgtagtcgtcccggttcaccttactgtgggactccgtctaacactcgttcgacttcctcgtcaagtt ENV > I I G T I R Q A H C N I S R A K W N D T L R Q I V S K L K E Q F K Sac1 Eco57 BstX1 SanD1 | | | gaacaagaccattgtgttcaaccagagctctgggggggaccctgagattgtgatgcacagcttcaactgtgggggggagttcttctactgcaacaccagc 4001 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 4100 cttgttctggtaacacaagttggtctcgagaccccccctgggactctaacactacgtgtcgaagttgacaccccccctcaagaagatgacgttgtggtcg ENV > N K T I V F N Q S S G G D P E I V M H S F N C G G E F F Y C N T S Pst1 Bts1 | | cccctgttcaacagcacctggaatggcaacaacacctggaacaacaccactggcagcaacaacaacatcaccctgcagtgcaagatcaagcagatcatca 4101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 4200 ggggacaagttgtcgtggaccttaccgttgttgtggaccttgttgtggtgaccgtcgttgttgttgtagtgggacgtcacgttctagttcgtctagtagt ENV > P L F N S T W N G N N T W N N T T G S N N N I T L Q C K I K Q I I N BspLU Bsg1 | | acatgtggcaggaggtgggcaaggccatgtatgccccccccattgagggccagatcaggtgcagcagcaacatcactggcctgctgctgaccagggatgg 4201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 4300 tgtacaccgtcctccacccgttccggtacatacggggggggtaactcccggtctagtccacgtcgtcgttgtagtgaccggacgacgactggtccctacc ENV > M W Q E V G K A M Y A P P I E G Q I R C S S N I T G L L L T R D G BsrG1 Bpm1 | | gggcaaggacactgacaccaatgacactgagattttcaggcctggggggggggacatgagggacaactggaggtctgagctgtacaagtacaaggtggtg 4301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 4400 cccgttcctgtgactgtggttactgtgactctaaaagtccggacccccccccctgtactccctgttgacctccagactcgacatgttcatgttccaccac ENV > G K D T D T N D T E I F R P G G G D M R D N W R S E L Y K Y K V V Apa1 Ear1 PspOM | Xcm1 BseR1| Bsg1 Bgl1| | | || | || | actattgagcccctgggggtggcccccaccaaggccaagaggagggtggtgcagagggagaagagggctgccattggggccctgttcctgggcttcctgg 4401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 4500 tgataactcggggacccccaccgggggtggttccggttctcctcccaccacgtctccctcttctcccgacggtaaccccgggacaaggacccgaaggacc ENV > T I E P L G V A P T K A K R R V V Q R E K R A A I G A L F L G F L G Bsg1 Bsg1 BbvC1 | | gggctgctggcagcactatgggggctgcctctgtgaccctgactgtgcaggccaggctgctgctgtctggcattgtgcagcagcagaacaacctgctgag 4501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 4600 cccgacgaccgtcgtgatacccccgacggagacactgggactgacacgtccggtccgacgacgacagaccgtaacacgtcgtcgtcttgttggacgactc ENV > A A G S T M G A A S V T L T V Q A R L L L S G I V Q Q Q N N L L R Bpu10 BseY1 BspM1 | Sfi1 | BstAP Pvu2 Kpn1 BfrB1 | Bgl1 | BstAP | Pst1| Pvu2 Pst1 Acc65 | | | | | | | || | | | | ggccattgaggcccagcagcacatgctgcagctgactgtgtggggcatcaagcagctgcaggccagggtgctggctgtggagaggtacctgaaggaccag 4601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 4700 ccggtaactccgggtcgtcgtgtacgacgtcgactgacacaccccgtagttcgtcgacgtccggtcccacgaccgacacctctccatggacttcctggtc ENV > A I E A Q Q H M L Q L T V W G I K Q L Q A R V L A V E R Y L K D Q Eco57 BseY1 | AlwN1 | Pvu2 | | Pvu2 AlwN1 | | Bsg1 Ale1 Bsm1 | Bpm1 | | | | | | | | cagctgctgggcttctggggctgctctggcaagctgatctgcaccaccactgtgccctggaatgccagctggagcaacaagagcctggatgacatctgga 4701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 4800 gtcgacgacccgaagaccccgacgagaccgttcgactagacgtggtggtgacacgggaccttacggtcgacctcgttgttctcggacctactgtagacct ENV > Q L L G F W G C S G K L I C T T T V P W N A S W S N K S L D D I W N Sap1 Bts1 Ear1 BseY1 Bpm1 | | | | acaacatgacctggatgcagtgggagagggagattgacaactacaccagcctgatctacagcctgctggagaagagccagacccagcaggagaagaatga 4801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 4900 tgttgtactggacctacgtcaccctctccctctaactgttgatgtggtcggactagatgtcggacgacctcttctcggtctgggtcgtcctcttcttact ENV > N M T W M Q W E R E I D N Y T S L I Y S L L E K S Q T Q Q E K N E BstAP PflM1 AlwN1 Bpm1 | BspH1 | | | | gcaggagctgctggagctggacaagtgggccagcctgtggaactggtttgacatcaccaactggctgtggtacatcaaaatcttcatcatgattgtgggg 4901 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 5000 cgtcctcgacgacctcgacctgttcacccggtcggacaccttgaccaaactgtagtggttgaccgacaccatgtagttttagaagtagtactaacacccc ENV > Q E L L E L D K W A S L W N W F D I T N W L W Y I K I F I M I V G BsmB1 Btr1 Bgl1Bsu36 Blp1| BmgB1BamH1 Bmr1 | | || | | | ggcctggtgggcctgaggattgtgtttgctgtgctgagctgagacgtgaggatccGAGGGTGAGTGCTCCTGCCTGGACGCATCCCGGCTATGCAGCCCC 5001 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 5100 ccggaccacccggactcctaacacaaacgacacgactcgactctgcactcctaggCTCCCACTCACGAGGACGGACCTGCGTAGGGCCGATACGTCGGGG ENV > G L V G L R I V F A V L S * orf 4 > M Q P Q Bpu10 Ear1 Bbs1 | | | | AGTCCAGGGCAGCAAGGCAGGCCCCGTCTGCCTCTTCACCCGGAGCCTCTGCCCGCCCCACTCATGCTCAGGGAGAGGGTCTTCTGGCTTTTTCCCAGGC 5101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 5200 TCAGGTCCCGTCGTTCCGTCCGGGGCAGACGGAGAAGTGGGCCTCGGAGACGGGCGGGGTGAGTACGAGTCCCTCTCCCAGAAGACCGAAAAAGGGTCCG orf 4 > S R A A R Q A P S A S S P G A S A R P T H A Q G E G L L A F S Q A BspM1 BseY1 BspM1 Bsg1 EcoN1 BfrB1 AlwN1 BfrB1 | | | | | TCTGGGCAGGCACAGGCTAGGTGCCCCTAACCCAGGCCCTGCACACAAAGGGGCAGGTGCTGGGCTCAGACCTGCCAAGAGCCATATCCGGGAGGACCCT 5201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 5300 AGACCCGTCCGTGTCCGATCCACGGGGATTGGGTCCGGGACGTGTGTTTCCCCGTCCACGACCCGAGTCTGGACGGTTCTCGGTATAGGCCCTCCTGGGA orf 4 > L G R H R L G A P N P G P A H K G A G A G L R P A K S H I R E D P BsaXb Bpu10 Bpu10 BbvC1 | BseR1 BsaXa | | | | | | GCCCCTGACCTAAGCCCACCCCAAAGGCCAAACTCTCCACTCCCTCAGCTCGGACACCTTCTCTCCTCCCAGATTCCAGTAACTCCCAATCTTCTCTCTG 5301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 5400 CGGGGACTGGATTCGGGTGGGGTTTCCGGTTTGAGAGGTGAGGGAGTCGAGCCTGTGGAAGAGAGGAGGGTCTAAGGTCATTGAGGGTTAGAAGAGAGAC orf 4 > A P D L S P P Q R P N S P L P Q L G H L L S S Q I P V T P N L L S A Pst1 Bpm1 BpuE1 | | | CAGAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGGTAAGCCAGCCCAGGCCTCGCCCTCCAGCTCAAGGCGGGACAGGTGCCCTAG 5401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 5500 GTCTCGGGTTTAGAACACTGTTTTGAGTGTGTACGGGTGGCACGGGTCCATTCGGTCGGGTCCGGAGCGGGAGGTCGAGTTCCGCCCTGTCCACGGGATC orf 4 > E P K S C D K T H T C P P C P G K P A Q A S P S S S R R D R C P R Ear1 BbvC1 Ahd1 Btr1 BsaXb | BsaXa | BseY1 BmgB1 Bpu10 | | AlwN1 Bbs1 | | | | | | | | | | | AGTAGCCTGCATCCAGGGACAGGCCCCAGCCGGGTGCTGACACGTCCACCTCCATCTCTTCCTCAGCACCTGAACTCCTGGGGGGACCGTCAGTCTTCCT 5501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 5600 TCATCGGACGTAGGTCCCTGTCCGGGGTCGGCCCACGACTGTGCAGGTGGAGGTAGAGAAGGAGTCGTGGACTTGAGGACCCCCCTGGCAGTCAGAAGGA orf 4 > V A C I Q G Q A P A G C * Drd1 Btr1 Bsu36 | Ear1 Bsu36 Ale1 BmgB1 Bbs1 | | | | | | | CTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTC 5601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 5700 GAAGGGGGGTTTTGGGTTCCTGTGGGAGTACTAGAGGGCCTGGGGACTCCAGTGTACGCACCACCACCTGCACTCGGTGCTTCTGGGACTCCAGTTCAAG orf 5 > M I S R T P E V T C V V V D V S H E D P E V K F BsaXa Sac2 BseR1 BsaXb Bsg1 | | | | | AACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGGGTGGTCAGCGTCCTCACCGTCC 5701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 5800 TTGACCATGCACCTGCCGCACCTCCACGTATTACGGTTCTGTTTCGGCGCCCTCCTCGTCATGTTGTCGTGCATGGCCCACCAGTCGCAGGAGTGGCAGG orf 5 > N W Y V D G V E V H N A K T K P R E E Q Y N S T Y R V V S V L T V L BsaXb EcoN1 BsaXa Bsa1| BseY1 | | || | TGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGTGG 5801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 5900 ACGTGGTCCTGACCGACTTACCGTTCCTCATGTTCACGTTCCAGAGGTTGTTTCGGGAGGGTCGGGGGTAGCTCTTTTGGTAGAGGTTTCGGTTTCCACC orf 5 > H Q D W L N G K E Y K C K V S N K A L P A P I E K T I S K A K G G Sfi1 Bgl1 SanD1 Nae1 | Bae1a Bae1b | | | | | GACCCGTGGGGTGCGAGGGCCACATGGACAGAGGCCGGCTCGGCCCACCCTCTGCCCTGAGAGTGACCGCTGTACCAACCTCTGTCCTACAGGGCAGCCC 5901 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 6000 CTGGGCACCCCACGCTCCCGGTGTACCTGTCTCCGGCCGAGCCGGGTGGGAGACGGGACTCTCACTGGCGACATGGTTGGAGACAGGATGTCCCGTCGGG orf 5 > T R G V R G P H G Q R P A R P T L C P E S D R C T N L C P T G Q P BspM1 BsrG1 Sma1 BfrB1 BseY1 | | | | CGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACA 6001 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 6100 GCTCTTGGTGTCCACATGTGGGACGGGGGTAGGGCCCTACTCGACTGGTTCTTGGTCCAGTCGGACTGGACGGACCAGTTTCCGAAGATAGGGTCGCTGT orf 5 > R E P Q V Y T L P P S R D E L T K N Q V S L T C L V K G F Y P S D I BsrD1 BsaXb BsaXa | | | TCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCT 6101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 6200 AGCGGCACCTCACCCTCTCGTTACCCGTCGGCCTCTTGTTGATGTTCTGGTGCGGAGGGCACGACCTGAGGCTGCCGAGGAAGAAGGAGATGTCGTTCGA orf 5 > A V E W E S N G Q P E N N Y K T T P P V L D S D G S F F L Y S K L BspM1 Sap1 BfrB1 Bbs1 Xmn1 Bsg1 Nsi1 Ear1 | | | | | | CACCGTGGACAAGAGCAGGTGGCAGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTG 6201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 6300 GTGGCACCTGTTCTCGTCCACCGTCGTCCCCTTGCAGAAGAGTACGAGGCACTACGTACTCCGAGACGTGTTGGTGATGTGCGTCTTCTCGGAGAGGGAC orf 5 > T V D K S R W Q Q G N V F S C S V M H E A L H N H Y T Q K S L S L Eag1 BspM1 | BfrB1 | Bpm1 BpuE1 | | | | TCTCCGGGTAAATGAGTGCGACGGCCGCAGGTAAGCCAGCCCAGGCCTCGCCCTCCAGCTCAAGGCGGGACAGGTGCCCTAGAGTAGCCTGCATCCAGGG 6301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 6400 AGAGGCCCATTTACTCACGCTGCCGGCGTCCATTCGGTCGGGTCCGGAGCGGGAGGTCGAGTTCCGCCCTGTCCACGGGATCTCATCGGACGTAGGTCCC orf 5 > S P G K * Btr1 Ear1 Bgl1 BseY1 BmgB1 Bsu36 Sma1 | Bmr1 Bpm1 | | | | | | | ACAGGCCCCAGCCGGGTGCTGACACGTCCACCTCCATCTCTTCCTCAGGTCTGCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGTCGT 6401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 6500 TGTCCGGGGTCGGCCCACGACTGTGCAGGTGGAGGTAGAGAAGGAGTCCAGACGGGCCCACCGTAGGGACACTGGGGAGGGGTCACGGAGAGGACCAGCA Ahd1 Ssp1 | | GGAAGGTGCTACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTTTGACTAGGTGTCCTTGTATAATATTATGGGGTGGAGG 6501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 6600 CCTTCCACGATGAGGTCACGGGTGGTCGGAACAGGATTATTTTAATTCAACGTAGTAAAACAAACTGATCCACAGGAACATATTATAATACCCCACCTCC Apa1 PspOM | Hpa1 Psi1 | | | | CGGGTGGTATGGAGCAAGGGGCCCAAGTTAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATT 6601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 6700 GCCCACCATACCTCGTTCCCCGGGTTCAATTGAACAAATAACGTCGAATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAA Bts1 Bsm1 Eco57 | | | TTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGATCGATCCGCTTCAGGCACCGGGCTTGCGGGTCATGCAC 6701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 6800 AAAAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAATAGTACAGACCTAGCTAGGCGAAGTCCGTGGCCCGAACGCCCAGTACGTG orf 6 < * A G P K R T M C Dra3 BstAP Aat2 BsrB1 Bsa1 | | | | CAGGTGCGCGGTCCTTCGGGCACCTCGACGTCGGCGGTGACGGTGAAGCCGAGCCGCTCGTAGAAGGGGAGGTTGCGGGGCGCGGAGGTCTCCAGGAAGG 6801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 6900 GTCCACGCGCCAGGAAGCCCGTGGAGCTGCAGCCGCCACTGCCACTTCGGCTCGGCGAGCATCTTCCCCTCCAACGCCCCGCGCCTCCAGAGGTCCTTCC orf 6 < W T R P G E P V E V D A T V T F G L R E Y F P L N R P A S T E L F BssH2 Eag1 Bcg1a Bcg1b BsmB1 BsaXb | | | | | | CGGGCACCCCGGCGCGCTCGGCCGCCTCCACTCCGGGGAGCACGACGGCGCTGCCCAGACCCTTGCCCTGGTGGTCGGGCGAGACGCCGACGGTGGCCAG 6901 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 7000 GCCCGTGGGGCCGCGCGAGCCGGCGGAGGTGAGGCCCCTCGTGCTGCCGCGACGGGTCTGGGAACGGGACCACCAGCCCGCTCTGCGGCTGCCACCGGTC orf 6 < A P V G A R E A A E V G P L V V A S G L G K G Q H D P S V G V T A L Nar1 Bgl1 | BsaXa| | Stu1 BsrB1 || | | | GAACCACGCGGGCTCCTTGGGCCGGTGCGGCGCCAGGAGGCCTTCCATCTGTTGCTGCGCGGCCAGCCTGGAACCGCTCAACTCGGCCATGCGCGGGCCG 7001 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 7100 CTTGGTGCGCCCGAGGAACCCGGCCACGCCGCGGTCCTCCGGAAGGTAGACAACGACGCGCCGGTCGGACCTTGGCGAGTTGAGCCGGTACGCGCCCGGC orf 6 < F W A P E K P R H P A L L G E M Q Q Q A A L R S G S L E A M R P G Nar1 Sac2 | Bgl1| | || | ATCTCGGCGAACACCGCCCCCGCTTCGACGCTCTCCGGCGTGGTCCAGACCGCCACCGCGGCGCCGTCGTCCGCGACCCACACCTTGCCGATGTCGAGCC 7101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 7200 TAGAGCCGCTTGTGGCGGGGGCGAAGCTGCGAGAGGCCGCACCAGGTCTGGCGGTGGCGCCGCGGCAGCAGGCGCTGGGTGTGGAACGGCTACAGCTCGG orf 6 < I E A F V A G A E V S E P T T W V A V A A G D D A V W V K G I D L Ear1 BstE2 BsrB1 Rsr2 Sal1 | | | | | CGACGCGCGTGAGGAAGAGTTCTTGCAGCTCGGTGACCCGCTCGATGTGGCGGTCCGGGTCGACGGTGTGGCGCGTGGCGGGGTAGTCGGCGAACGCGGC 7201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 7300 GCTGCGCGCACTCCTTCTCAAGAACGTCGAGCCACTGGGCGAGCTACACCGCCAGGCCCAGCTGCCACACCGCGCACCGCCCCATCAGCCGCTTGCGCCG orf 6 < G V R T L F L E Q L E T V R E I H R D P D V T H R T A P Y D A F A A Aat2 BsiW1 Sma1 PflF1| Pst1 | | || | GGCGAGGGTGCGTACGGCCCGGGGGACGTCGTCGCGGGTGGCGAGGCGCACCGTGGGCTTGTACTCGGTCATGGTGGCCTGCAGAGTCGCTCTGTGTTCG 7301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 7400 CCGCTCCCACGCATGCCGGGCCCCCTGCAGCAGCGCCCACCGCTCCGCGTGGCACCCGAACATGAGCCAGTACCACCGGACGTCTCAGCGAGACACAAGC orf 6 < A L T R V A R P V D D R T A L R V T P K Y E T M Mlu1 BseY1 | | AGGCCACACGCGTCACCTTAATATGCGAAGTGGACCTGGGACCGCGCCGCCCCGACTGCATCTGCGTGTTTTCGCCAATGACAAGACGCTGGGCGGGGTT 7401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 7500 TCCGGTGTGCGCAGTGGAATTATACGCTTCACCTGGACCCTGGCGCGGCGGGGCTGACGTAGACGCACAAAAGCGGTTACTGTTCTGCGACCCGCCCCAA Pvu2 | TGTGTCATCATAGAACTAAAGACATGCAAATATATTTCTTCCGGGGACACCGCCAGCAAACGCGAGCAACGGGCCACGGGGATGAAGCAGCTGCGCCACT 7501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 7600 ACACAGTAGTATCTTGATTTCTGTACGTTTATATAAAGAAGGCCCCTGTGGCGGTCGTTTGCGCTCGTTGCCCGGTGCCCCTACTTCGTCGACGCGGTGA Eco57 BsmB1 | | | CCCTGAAGATCCATCGTCTCCTAACAAGTTACATCACTCCTGCCCTTCCTCACCCTCATCTCCATCACCTCCTTCATCTCCGTCATCTCCGTCATCACCC 7601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 7700 GGGACTTCTAGGTAGCAGAGGATTGTTCAATGTAGTGAGGACGGGAAGGAGTGGGAGTAGAGGTAGTGGAGGAAGTAGAGGCAGTAGAGGCAGTAGTGGG orf 7 < * E Q G E E G E D G D G G E D G D D G D D G BspM1 Sac2 Bsg1 BfrB1 BsrB1 | | | | TCCGCGGCAGCCCCTTCCACCATAGGTGGAAACCAGGGAGGCAAATCTACTCCATCGTCAAAGCTGCACACAGTCACCCTGATATTGCAGGTAGGAGCGG 7701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 7800 AGGCGCCGTCGGGGAAGGTGGTATCCACCTTTGGTCCCTCCGTTTAGATGAGGTAGCAGTTTCGACGTGTGTCAGTGGGACTATAACGTCCATCCTCGCC orf 7 < E A A A G E V M P P F W P P L D V G D D F S C V T V R I N C T P A Bpu10 BbvC1 Bpu10 | | | GCTTTGTCATAACAAGGTCCTTAATCGCATCCTTCAAAACCTCAGCAAATATATGAGTTTGTAAAAAGACCATGAAATAACAGACAATGGACTCCCTTAG 7801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 7900 CGAAACAGTATTGTTCCAGGAATTAGCGTAGGAAGTTTTGGAGTCGTTTATATACTCAAACATTTTTCTGGTACTTTATTGTCTGTTACCTGAGGGAATC orf 7 < P K T M V L D K I A D K L V E A F I H T Q L F V M F Y C V I S E R L BsmB1 Bsu36 | | CGGGCCAGGTTGTGGGCCGGGTCCAGGGGCCATTCCAAAGGGGAGACGACTCAATGGTGTAAGACGACATTGTGGAATAGCAAGGGCAGTTCCTCGCCTT 7901 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 8000 GCCCGGTCCAACACCCGGCCCAGGTCCCCGGTAAGGTTTCCCCTCTGCTGAGTTACCACATTCTGCTGTAACACCTTATCGTTCCCGTCAAGGAGCGGAA orf 7 < P G P Q P G P G P A M G F P L R S L P T L R C Q P I A L A T G R R SgrA1 | AGGTTGTAAAGGGAGGTCTTACTACCTCCATATACGAACACACCGGCGACCCAAGTTCCTTCGTCGGTAGTCCTTTCTACGTGACTCCTAGCCAGGAGAG 8001 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 8100 TCCAACATTTCCCTCCAGAATGATGGAGGTATATGCTTGTGTGGCCGCTGGGTTCAAGGAAGCAGCCATCAGGAAAGATGCACTGAGGATCGGTCCTCTC orf 7 < L N Y L S T K S G G Y V F V G A V W T G E D T T R E V H S R A L L Sac1 BsrD1 | | CTCTTAAACCTTCTGCAATGTTCTCAAATTTCGGGTTGGAACCTCCTTGACCACGATGCTTTCCAAACCACCCTCCTTTTTTGCGCCTGCCTCCATCACC 8101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 8200 GAGAATTTGGAAGACGTTACAAGAGTTTAAAGCCCAACCTTGGAGGAACTGGTGCTACGAAAGGTTTGGTGGGAGGAAAAAACGCGGACGGAGGTAGTGG orf 7 < A R L G E A I N E F K P N S G G Q G R H K G F W G G K K R R G G D G Btr1 BmgB1 | CTGACCCCCGCTGCGCGGGGGCACGTCAGGCTCACCATCTGGGCCGCCTTCTTGGTGGTATTCAAAATAATCGGCTTCCCCTACAGGGTGGAAAAATGGC 8201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 8300 GACTGGGGGCGACGCGCCCCCGTGCAGTCCGAGTGGTAGACCCGGCGGAAGAACCACCATAAGTTTTATTAGCCGAAGGGGATGTCCCACCTTTTTACCG orf 7 < Q G G S R P P V D P E G D P G G E Q H Y E F Y D A E G V P H F F P BsaXb Btr1 Bsa1 BsaXb Bmr1 BsaXa |BmgB1 | | | | | | CTTCTACCTGGAGGGGGCCTGCGCGGTGGAGACCCGGATGATGATGACTGACTACTGGGACTCCTGGGCCTCTTTTCTCCACGTCCACGACCTCTCCCCC 8301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 8400 GAAGATGGACCTCCCCCGGACGCGCCACCTCTGGGCCTACTACTACTGACTGATGACCCTGAGGACCCGGAGAAAAGAGGTGCAGGTGCTGGAGAGGGGG orf 7 < R R G P P P R R P P S G S S S S Q S S P S R P R K E G R G R G R G Btr1 BsaXa BmgB1 BseR1 BseR1 | | | | TGGCTCTTTCACGACTTCCCCCCCTGGCTCTTTCACGTCCTCTACCCCGGCGGCCTCCACTACCTCCTCGACCCCGGCCTCCACTACCTCCTCGACCCCG 8401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 8500 ACCGAGAAAGTGCTGAAGGGGGGGACCGAGAAAGTGCAGGAGATGGGGCCGCCGGAGGTGATGGAGGAGCTGGGGCCGGAGGTGATGGAGGAGCTGGGGC orf 7 < R A R E R S G G R A R E R G R G R R G G S G G R G R G G S G G R G R Bts1 BseR1 | BsrB1 SanD1 BstAP | | | | | GCCTCCACTGCCTCCTCGACCCCGGCCTCCACCTCCTGCTCCTGCCCCTCCCGCTCCTGCTCCTGCTCCTGTTCCACCGTGGGTCCCTTTGCAGCCAATG 8501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 8600 CGGAGGTGACGGAGGAGCTGGGGCCGGAGGTGGAGGACGAGGACGGGGAGGGCGAGGACGAGGACGAGGACAAGGTGGCACCCAGGGAAACGTCGGTTAC orf 7 < G G S G G R G R G G G G A G A G G A G A G A G T G G H T G K C G I Eci1 Bsa1 Bbs1| BspE1 | Bpu10 Sma1 || BseR1 | | | | || | CAACTTGGACGTTTTTGGGGTCTCCGGACACCATCTCTATGTCTTGGCCCTGATCCTGAGCCGCCCGGGGCTCCTGGTCTTCCGCCTCCTCGTCCTCGTC 8601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 8700 GTTGAACCTGCAAAAACCCCAGAGGCCTGTGGTAGAGATACAGAACCGGGACTAGGACTCGGCGGGCCCCGAGGACCAGAAGGCGGAGGAGCAGGAGCAG orf 7 < C S P R K Q P R R V G D R H R P G S G S G G P A G P R G G G R G R Pvu2 Ear1 Bgl1 Pst1| | | || CTCTTCCCCGTCCTCGTCCATGTGCCATGATGGCGGCCTGCAGCTGTGTTCGAGGCCGCGCGTGTCACCTTAATATGCGAAGTGGACCTGGGACCGCGCC 8701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 8800 GAGAAGGGGCAGGAGCAGGTACACGGTACTACCGCCGGACGTCGACACAAGCTCCGGCGCGCACAGTGGAATTATACGCTTCACCTGGACCCTGGCGCGG orf 7 < G R G R G R G H A M BseY1 Nde1 Sma1 | | | GCCCCGACTGCATCTGCGTGTTCGAGTTCGCCAATGACAAGACGCTGGGCGGGGAGATCCCCCTTATTAACCCTAAACGGGTAGCATATGCTTCCCGGGT 8801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 8900 CGGGGCTGACGTAGACGCACAAGCTCAAGCGGTTACTGTTCTGCGACCCGCCCCTCTAGGGGGAATAATTGGGATTTGCCCATCGTATACGAAGGGCCCA Nde1 Nde1 Bsu36 | | | AGTAGTATATACTATCCAGACTAACCCTAATTCAATAGCATATGTTACCCAACGGGAAGCATATGCTATCGAATTAGGGTTAGTAAAAGGGTCCTAAGGA 8901 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 9000 TCATCATATATGATAGGTCTGATTGGGATTAAGTTATCGTATACAATGGGTTGCCCTTCGTATACGATAGCTTAATCCCAATCATTTTCCCAGGATTCCT Nde1 Nde1 | | ACAGCGATCTGGATAGCATATGCTATCCTAATCTATATCTGGGTAGCATATGCTATCCTAATCTATATCTGGGTAGCATAGGCTATCCTAATCTATATCT 9001 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 9100 TGTCGCTAGACCTATCGTATACGATAGGATTAGATATAGACCCATCGTATACGATAGGATTAGATATAGACCCATCGTATCCGATAGGATTAGATATAGA Nde1 | GGGTAGCATATGCTATCCTAATCTATATCTGGGTAGTATATGCTATCCTAATTTATATCTGGGTAGCATAGGCTATCCTAATCTATATCTGGGTAGCATA 9101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 9200 CCCATCGTATACGATAGGATTAGATATAGACCCATCATATACGATAGGATTAAATATAGACCCATCGTATCCGATAGGATTAGATATAGACCCATCGTAT Bts1 BseR1 BseR1 BseR1 | | | | | CGGCCTCCACTACCTCCTCGACCCCGGCCTCCACTACCTCCTCGACCCCGGCCTCCACTGCCTCCTCGACCCCGGCCTCCACCTCCTGCTCCTGCCCCTC 9201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 9300 GCCGGAGGTGATGGAGGAGCTGGGGCCGGAGGTGATGGAGGAGCTGGGGCCGGAGGTGACGGAGGAGCTGGGGCCGGAGGTGGAGGACGAGGACGGGGAG Bsa1 BsrB1 SanD1 BstAP BspE1 | Bpu10 | | | | | | CCGCTCCTGCTCCTGCTCCTGTTCCACCGTGGGTCCCTTTGCAGCCAATGCAACTTGGACGTTTTTGGGGTCTCCGGACACCATCTCTATGTCTTGGCCC 9301 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 9400 GGCGAGGACGAGGACGAGGACAAGGTGGCACCCAGGGAAACGTCGGTTACGTTGAACCTGCAAAAACCCCAGAGGCCTGTGGTAGAGATACAGAACCGGG Eci1 Bbs1| Pvu2 Sma1 || BseR1 Ear1 Bgl1 Pst1| | || | | | || TGATCCTGAGCCGCCCGGGGCTCCTGGTCTTCCGCCTCCTCGTCCTCGTCCTCTTCCCCGTCCTCGTCCATGTGCCATGATGGCGGCCTGCAGCTGTGTT 9401 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 9500 ACTAGGACTCGGCGGGCCCCGAGGACCAGAAGGCGGAGGAGCAGGAGCAGGAGAAGGGGCAGGAGCAGGTACACGGTACTACCGCCGGACGTCGACACAA BseY1 | CGAGGCCGCGCGTGTCACCTTAATATGCGAAGTGGACCTGGGACCGCGCCGCCCCGACTGCATCTGCGTGTTCGAGTTCGCCAATGACAAGACGCTGGGC 9501 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 9600 GCTCCGGCGCGCACAGTGGAATTATACGCTTCACCTGGACCCTGGCGCGGCGGGGCTGACGTAGACGCACAAGCTCAAGCGGTTACTGTTCTGCGACCCG Nde1 Sma1 Nde1 | | | GGGGAGATCCCCCTTATTAACCCTAAACGGGTAGCATATGCTTCCCGGGTAGTAGTATATACTATCCAGACTAACCCTAATTCAATAGCATATGTTACCC 9601 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 9700 CCCCTCTAGGGGGAATAATTGGGATTTGCCCATCGTATACGAAGGGCCCATCATCATATATGATAGGTCTGATTGGGATTAAGTTATCGTATACAATGGG Nde1 Bsu36 Nde1 Nde1 | | | | AACGGGAAGCATATGCTATCGAATTAGGGTTAGTAAAAGGGTCCTAAGGAACAGCGATCTGGATAGCATATGCTATCCTAATCTATATCTGGGTAGCATA 9701 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 9800 TTGCCCTTCGTATACGATAGCTTAATCCCAATCATTTTCCCAGGATTCCTTGTCGCTAGACCTATCGTATACGATAGGATTAGATATAGACCCATCGTAT Nde1 | TGCTATCCTAATCTATATCTGGGTAGCATAGGCTATCCTAATCTATATCTGGGTAGCATATGCTATCCTAATCTATATCTGGGTAGTATATGCTATCCTA 9801 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 9900 ACGATAGGATTAGATATAGACCCATCGTATCCGATAGGATTAGATATAGACCCATCGTATACGATAGGATTAGATATAGACCCATCATATACGATAGGAT Nde1 | ATTTATATCTGGGTAGCATAGGCTATCCTAATCTATATCTGGGTAGCATATGCTATCCTAATCTATATCTGGGTAGTATATGCTATCCTAATCTGTATCC 9901 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 10000 TAAATATAGACCCATCGTATCCGATAGGATTAGATATAGACCCATCGTATACGATAGGATTAGATATAGACCCATCATATACGATAGGATTAGACATAGG Nde1 Bmr1 BciV1 | Nsi1 Nde1 | Xcm1 Nde1 Bgl2 | | | | | | | | GGGTAGCATATGCTATCCTCATGCATATACAGTCAGCATATGATACCCAGTAGTAGAGTGGGAGTGCTATCCTTTGCATATGCCGCCACCTCCCAAGGAG 10001 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 10100 CCCATCGTATACGATAGGAGTACGTATATGTCAGTCGTATACTATGGGTCATCATCTCACCCTCACGATAGGAAACGTATACGGCGGTGGAGGGTTCCTC BsrG1 Eci1 Sal1 Cla1 Eci1 | Eci1 Bmr1| Eci1 | | | | | || | ATCTGTCGACATCGATGGGCGCGGGTGTACACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCTCATGGCTGACTAATTTTTT 10101 ---------+---------+---------+---------+---------+---------+---------+---------+---------+---------+ 10200 TAGACAGCTGTAGCTACCCGCGCCCACATGTGAGGCGGGTAGGGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAGGCGGAGTACCGACTGATTAAAAAA Avr2 Sfi1 Stu1| Bgl1 BseR1 || | | || TTATTTATGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTAATTC 10201 ---------+---------+---------+---------+---------+---------+---------+---------+---------+--------- 10299 AATAAATACGTCTCCGGCTCCGGCGGAGCCGGAGACTCGATAAGGTCTTCATCACTCCTCCGAAAAAACCTCCGGATCCGAAAACGTTTTTCGATTAAGFound: Aat2 Acc65 Acl1 Afl2 Age1 Ahd1 Ale1 AlwN1 Apa1 ApaL1 Avr2 Bae1a Bae1b BamH1 Bbs1 BbvC1 Bcg1a Bcg1b BciV1 BfrB1 Bgl1 Bgl2 Blp1 BmgB1 Bmr1 Bpm1 Bpu10 BpuE1 Bsa1 BsaXa BsaXb BseR1 BseY1 Bsg1 BsiW1 Bsm1 BsmB1 BspE1 BspH1 BspLU BspM1 BsrB1 BsrD1 BsrG1 BssH2 BssS1 BstAP BstE2 BstX1 Bsu36 Btr1 Bts1 Cla1 Dra1 Dra3 Drd1 Eag1 Ear1 Eci1 Eco57 EcoK EcoN1 Fse1 Hind3 Hpa1 Kpn1 Mfe1 Mlu1 Msc1 Nae1 Nar1 Nco1 Nde1 Nhe1 Nsi1 PflF1 PflM1 Pme1 Psi1 PspOM Pst1 Pvu2 Rsr2 Sac1 Sac2 Sal1 SanD1 Sap1 Sfi1 SgrA1 Sma1 Spe1 Ssp1 Stu1 Xba1 Xcm1 Xho1 Xmn1 Not found: Afe1 Asc1 Ase1 AsiS1 Bcl1 BsaB1 BstB1 BstZ1 EcoR1 EcoRV Fsp1 I_Ceu loxP Not1 Nru1 Pac1 Pml1 PshA1 Pvu1 Sbf1 Sca1 SexA1 Sgf1 SnaB1 Sph1 Srf1 Swa1 Unique: Acc65 Afl2 Age1 Avr2 Bae1a Bae1b BamH1 Bgl2 Blp1 BsiW1 BspH1 BssH2 BstE2 Cla1 EcoK Fse1 Hind3 Hpa1 Kpn1 Mfe1 Mlu1 Msc1 Nco1 Nhe1 PflF1 PflM1 Pme1 Psi1 Rsr2 SgrA1 Spe1 Xba1 Excluded by site complexity: Acc1 Aci1 Afl3 Alu1 Alw1 Apo1 Ava1 Ava2 Ban1 Ban2 Bbv1 BceA1 Bfa1 Bme15 BsaA1 BsaH1 BsaJ1 BsaW1 BseM2 BseS1 BsiE1 BsiH1 Bsl1 BsmA1 BsmF1 Bsp12 BspCa BspCb Bsr1 BsrF1 BssK1 BstF5 BstN1 BstU1 BstY1 Btg1 Cac8 CviJ1 Dde1 Eae1 EcoO1 Fau1 Fnu4H Fok1 Hae2 Hae3 Hga1 Hha1 Hinc2 Hinf1 HinP1 Hpa2 Hph1 Hpy99 Hpy1 Hpy3 HpyC3 HpyC4 HpyC5 Mae3 Mbo2 Mnl1 Mse1 Msl1 MspA1 Mwo1 Nci1 Nla3 Nla4 Nsp1 Ple1 PpuM1 Rsa1 Sau3A Sau96 SfaN1 Sfc1 Sml1 Sty1 Taq1 Tat1 Tfi1 Tse1 Tsp45 Tsp50 TspR1