Resources
Protocols
Oligomerized Pool ENgineering (OPEN) method for constructing zinc finger arrays (link to be added when published at end of August)
Modular assembly method for engineering zinc finger arrays http://www.nature.com/nprot/journal/v1/n3/abs/nprot.2006.259.html
High-throughput beta-galactosidase assay http://www.zincfingers.org/jounglab/b-galProtocolJoungLab.doc
Plasmids, strains, and reagents
Oligomerized Pool ENgineering (OPEN) reagents http://www.addgene.org/pgvec1?f=c&cmd=showcol&colid=235&page=5
Modular assembly engineering reagents http://www.addgene.org/pgvec1?f=c&cmd=showcol&colid=235&page=1
Zinc Finger Nuclease (ZFN) expression vectors(http://www.addgene.org/pgvec1?f=c&cmd=showcol&colid=235&page=7
Bacterial two-hybrid system vector maps and sequences http://www.zincfingers.org/jounglab/B2H_plasmid_mapsJoungLab.doc
Software
ZiFiT (Zinc Finger Targeter; web-based software for designing zinc finger arrays using modular design, Dobbs and Voytas labs, Iowa State University) http://bindr.gdcb.iastate.edu/ZiFiT/
ZiFDB (Zinc Finger Database; web-accessible database of zinc fingers and engineered zinc finger arrays, Voytas laboratory, University of Minnesota) http://bindr.gdcb.iastate.edu:8080/ZiFDB/
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